Journal: Molecular Oncology

Article Title: A single lysis solution for the analysis of tissue samples by different proteomic technologies

doi: 10.1016/j.molonc.2008.09.003

Figure Lengend Snippet: Two‐dimensional gel electrophoresis protein patterns of whole protein extracts prepared from a needle core biopsy collected from a breast ductal carcinoma. Proteins were solubilized in O'Farrell lysis solution (A) or in CLB1 solution (B). Proteins ...

Article Snippet: The resulting lysate solutions were then serially 2‐fold diluted three times with CLB1 buffer and transferred onto FAST nitrocellulose microarray slides (Schleicher & Schuell BioScience, Germany) in quintuplicates using a MicroCaster™ Hand‐Held Microarrayer System (Schleicher & Schuell BioScience, Germany) according to manufacturer's instructions.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Lysis

Journal: Molecular Oncology

Article Title: A single lysis solution for the analysis of tissue samples by different proteomic technologies

doi: 10.1016/j.molonc.2008.09.003

Figure Lengend Snippet: IEF 2D gel of whole tissue proteins extracted with O'Farrell lysis solution (left‐hand images) or CLB1 lysis solution (right‐hand images) from human brain cortex, cerebellum, kidney cortex, skeletal muscle and tongue. Solubilization of ...

Article Snippet: The resulting lysate solutions were then serially 2‐fold diluted three times with CLB1 buffer and transferred onto FAST nitrocellulose microarray slides (Schleicher & Schuell BioScience, Germany) in quintuplicates using a MicroCaster™ Hand‐Held Microarrayer System (Schleicher & Schuell BioScience, Germany) according to manufacturer's instructions.

Techniques: Two-Dimensional Gel Electrophoresis, Lysis

Journal: Molecular Oncology

Article Title: A single lysis solution for the analysis of tissue samples by different proteomic technologies

doi: 10.1016/j.molonc.2008.09.003

Figure Lengend Snippet: IPG‐2D PAGE of whole protein extracts from a breast carcinoma sample run using IPG 4–7 (A) and 5–8 (B) strips. Proteins were solubilized in IPG lysis solution (BioRad) (left‐hand panel) or in CLB1 solution (right‐hand ...

Article Snippet: The resulting lysate solutions were then serially 2‐fold diluted three times with CLB1 buffer and transferred onto FAST nitrocellulose microarray slides (Schleicher & Schuell BioScience, Germany) in quintuplicates using a MicroCaster™ Hand‐Held Microarrayer System (Schleicher & Schuell BioScience, Germany) according to manufacturer's instructions.

Techniques: Lysis

Journal: Molecular Oncology

Article Title: A single lysis solution for the analysis of tissue samples by different proteomic technologies

doi: 10.1016/j.molonc.2008.09.003

Figure Lengend Snippet: Compatibility of the CLB1 solution with the antibody protein microarray format. The tissue sample was frozen‐sectioned and solubilized in either Panorama® Antibody Microarray Extraction buffer or in CLB1 lysis solution and labeled with ...

Article Snippet: The resulting lysate solutions were then serially 2‐fold diluted three times with CLB1 buffer and transferred onto FAST nitrocellulose microarray slides (Schleicher & Schuell BioScience, Germany) in quintuplicates using a MicroCaster™ Hand‐Held Microarrayer System (Schleicher & Schuell BioScience, Germany) according to manufacturer's instructions.

Techniques: Microarray, Lysis, Labeling

Journal: Molecular Oncology

Article Title: A single lysis solution for the analysis of tissue samples by different proteomic technologies

doi: 10.1016/j.molonc.2008.09.003

Figure Lengend Snippet: Compatibility of the CLB1 solution with the antibody protein microarray format. The tissue sample was frozen‐sectioned and solubilized in either Panorama® Antibody Microarray Extraction buffer or in CLB1 lysis solution and labeled with ...

Article Snippet: The resulting lysate solutions were then serially 2‐fold diluted three times with CLB1 buffer and transferred onto FAST nitrocellulose microarray slides (Schleicher & Schuell BioScience, Germany) in quintuplicates using a MicroCasterTM Hand‐Held Microarrayer System (Schleicher & Schuell BioScience, Germany) according to manufacturer's instructions.

Techniques: Microarray, Extraction, Lysis, Labeling